Fig. 4
From: Inhibition of p90 ribosomal S6 kinases disrupts melanoma cell growth and immune evasion
RSK inhibition promotes pigmentation and the expression of melanocyte differentiation antigens in melanoma cells. A, B Photographs of SKMel30 (NRASMut) melanoma cells (A: cell pellet after 72 h two-dimensional culture; B: 10 d spheroid culture on a 24-well plate) treated with increasing doses of PMD-026. C Real-time qPCR analysis for melanocyte differentiation antigen (MDA) transcript expression in melanoma cell lines of different mutational subgroups (NRASMut: green, NF-1LOF: blue, BRAFMut: red) and a patient-derived short-term culture (yellow) after RSK inhibition (PMD-026 [5 µM], 72 h). RPLP0 and POLR2A (RNA Polymerase II) were used as reference genes. Fold change induction by RSK inhibition was visualized by normalization to the respective solvent-treated control cells (mean ± SD; N ≥ 2 with n = 2). D Western blot analysis of MDAs in whole cell protein lysates after treatment with PMD-026 or its solvent control DMSO for 72 h. Vinculin served as loading control