Fig. 4

Fructose regulates the function of VECs through AKT and Src signaling pathways. a and b, Western blot analysis was performed to evaluate the activation of Akt and Src signaling pathways in SVEC4-10 (a) and HUVEC (b) cells cultured for 6 h under the indicated conditions, and the relative expression of the proteins was analyzed by grayscale using Image J. c and d, The viability of SVEC4-10 (c) and HUVEC (d) cells was analyzed by CCK-8 in fructose (20 mM) medium with the addition of the Akt pathway inhibitor MK2206 (6 μM) or the Src pathway inhibitor Dasatinib (0.3 μM) for 48 h. e, Tube-forming ability of SVEC4-10 cells cultured in fructose medium containing MK2206 (6 μM) or Dasatinib (0.3 μM). Scale bar: 50 μm. f, Budding ability of isolated rat thoracic aortic rings in fructose medium containing MK2206 (6 μM) or Dasatinib (0.3 μM). Scale bar: 500 μm. All data are expressed as the mean ± SD; ns, non-significant; *p < 0.05; **p < 0.01