Fig. 6

Effect of fructose-conditioned culture medium of tumor cells on the biological function of VECs. a, The relationship between Glut5 expression levels and MVD in colorectal cancer tissues (n = 448) was analyzed by IHC staining. Scale bar: 50 μm. b, GSEA analysis identified up- and down-regulated pathways in tissues with high Glut5 mRNA expression based on the TCGA colorectal cancer dataset. c, The positive correlation between angiogenesis and Glut5 mRNA. d, Correlation of Glut5 mRNA levels with 47 angiogenesis-related genes based on the TCGA colorectal cancer dataset, and the 47 angiogenesis-related genes were obtained in MisigDB under the HALLMARK geneset. e, Schematic description showing the collection of different conditional media. CM1 was fructose-containing conditional medium, and CM2 was conditional medium without fructose. f, Effects of the CM on the proliferation of SVEC4-10 cells. g and h, Effects of the CM1 (g) and CM2 (h) on the migration and tube-forming abilities of SVEC4-10 cells, and the budding ability of rat aortic rings. Scale bar: 100, 50 and 500 μm, respectively. All data are expressed as the mean ± SD; ns, non-significant; *p < 0.05; **p < 0.01