Fig. 4

Expression, prognostic value and underlying mechanisms of SNHG4 in prostate cancer. Analysis based on the TCGA_PRAD dataset suggested that SNHG4 is highly expressed in PCa tumor tissues (a) and is closely correlated with T stage (b) and high Gleason score (c) of the tumors. d Kaplan‒Meier plot showing that high SNHG4 expression is positively related to poor OS in PCa patients (p = 0.035, HR = 6.64). The data were analyzed based on the TCGA_PRAD dataset. e Expression of SNHG4 was analyzed in 30 BPH tissues and 60 pairs of PCa and adjacent normal tissue samples by qRT‒PCR. f Expression of SNHG4 was analyzed and compared between the low Gleason score group (n = 37) and the high Gleason score group (n = 23). g Representative images showing SNHG4 expression in BPH tissues, PCa tissues with Gleason score < 7 and PCa tissues with Gleason score > 7 measured by ISH staining. Magnification: 200X. h Chord diagram indicates the enriched biological processes and pathways of SNHG4 in prostate cancer. Gene set enrichment analysis suggested that SNHG4-correlated genes were enriched in biological processes such as “G1_S_Specific_Transcription” (i) and “Oxidative_Stress_Induced_Senescence” (j). k Mountain diagram shows some key biological processes in which SNHG4-correlated genes were enriched in prostate cancer. l Subcellular locations of SNHG4 in different cell lines were predicted by LncATLAS tools. The localization of lncRNAs was calculated as the cytoplasmic/nuclear relative concentration index (CN RCI). m Representative images of the subcellular location of SNHG4 in 22Rv1 and LNCaP cells by immunostaining. Scale bar = 20 μM. ns indicates not significant, * indicates p < 0.05, ** indicates p < 0.01, *** indicates p < 0.001