Fig. 5

The cell cycle regulators EZH2/AURKA/TK1 are targeted by let-7 miRNAs and regulated by SNHG4 via a ceRNA network. a GO/KEGG analysis showed the enriched biological terms of the 40 genes that were overexpressed across three GEO datasets. b Venn diagram showing that RRM2, EZH2, AURAK and TK1 were overexpressed across the three GEO datasets and potential target genes of let-7 miRNAs. c SNHG4, RRM2, EZH2, AURKA and TK1 were coexpressed genes in PCa tumor tissues, and the data were analyzed based on the TCGA_PRAD dataset. d The sequences of predicted binding sites between SNHG4, let-7 miRNAs and EZH2, AURKA and TK1 are shown. e, f and g SNHG4 was overexpressed or knocked down in 22Rv1 and LNCaP cells, and the expression of EZH2, AURKA and TK1 was measured by qRT‒PCR and western blotting. h, i and j Expression of EZH2, AURKA and TK1 was measured in control cells, SNHG4-overexpressing cells or SNHG4 and let-7a double-overexpressing cells by qRT‒PCR and western blot. k, l and m Expression of EZH2, AURKA and TK1 was measured in control/let-7a-overexpressing or scramble/let-7a-knockdown PCa cells by qRT‒PCR and western blot. n RIP assay indicating the enrichment of SNHG4, let-7a, EZH2, AURKA and TK1 in the RNA products pulled down by anti-IgG/anti-AGO antibody before or after SNHG4 overexpression. ns indicates not significant, * indicates p < 0.05, ** indicates p < 0.01