Fig. 6

Depletion of SNHG4, RRM2, EZH2, AURKA or TK1 suppresses cell viability and induces senescence and SASP in PCa cells. a and b EZH2, AURKA or TK1 expression was knocked down in PCa cell lines, and the CCK-8 assay showed the proliferation curve of the cells. c and d EZH2, AURKA or TK1 was knocked down in 22Rv1 and LNCaP cells, and after 48 h, the cells were subjected to EdU staining. Numbers of proliferative cells were counted by ImageJ. e and f Cell proliferation was assessed by colony formation assay in EZH2, AURKA or TK1 expression knockdown PCa cell lines. g Representative images of 22Rv1 cells after SA-β-Gal staining. SNHG4, RRM2, EZH2, AURKA or TK1 was depleted in 22Rv1 cells, and cell senescence was measured by SA-β-Gal assay. Blue color indicated the positively stained cells, which were senescent cells. Magnification: 200X. h The ATP content was measured in SNHG4-, RRM2-, EZH2-, AURKA- or TK1-depleted PCa cells. i Expression of SASP markers was measured in SNHG4-, RRM2-, EZH2-, AURKA- or TK1-depleted PCa cells by qRT‒PCR. ns indicates not significant, * indicates p < 0.05, ** indicates p < 0.01