Fig. 7

The SNHG4/let-7a/RRM2 axis modulates the cell viability, cell cycle and DNA damage response of PCa cells. CCK-8 assay (a), EdU assay (b) and colony formation assay (c) were performed to measure cell proliferation in control cells, SNHG4-depleted cells, SNHG4 and let-7a double-depleted cells or SNHG4-depleted with RRM2 reoverexpressed cells. d γ-H2AX foci were measured by immunostaining assay in four groups of experimental cells 24 h after DTX treatment (10 nM). e The cell cycle was measured by FACS in four groups of experimental cells 48 h after treatment. f In vivo tumor lumps removed from four groups of CDX mice. g The tumor growth curves for in vivo tumor volumes. h The mean tumor weight of each group. i SNHG4 knockdown reduced Ki67 expression in xenograft tumors, whereas simultaneous let-7a knockdown or RRM2 reoverexpression rescued Ki67 expression, as shown by IHC staining. Magnification: 200X. ns indicates not significant, * indicates p < 0.05, ** indicates p < 0.01