Fig. 2

Knockdown of METTL1 inhibits CRPC cell proliferation and invasion. A-B Detection of METTL1 mRNA and protein level between the HSPC and CRPC cell lines. C-E METTL1 knockdown efficiency in LNCaP-AI and C4-2 cells via RT-qPCR and western blot. F-K Validation of proliferation ability change after METTL1 was inhibited in LNCaP-AI and C4-2 cells through MTT (F, G) and colony formation (H-K) assays. L-O Transwell was performed to determine the invasion capacity variation of LNCaP-AI and C4-2 cells caused by METTL1 suppression. Three biological replicates of each experiment were performed. ***P < 0.001; ****P < 0.0001