Fig. 6

METTL1 regulates CRPC proliferation and invasion by modulating the expression of CDK14. A Validation of CDK14 expression in TMA containing HSPC and CRPC tissues using IHC assay. Objective magnification: 40X, 100X, and 200X. B The proportion of patients with different CDK14 levels according to the IHC staining result. C The different mRNA expressions of CDK14 between HSPC and CRPC cell lines by RT-qPCR. D-F RT-qPCR and western blot were performed to detect the CDK14 level after METTL1 was rescued and inhibition of CDK14 using siRNA or not. G-I Proliferation ability of LNCaP-AI and C4-2 were assessed through MTT and colony formation experiments after METTL1 was rescued and CDK14 was suppressed. J Invasion capacity of LNCaP-AI and C4-2 were evaluated via transwell experiments after METTL1 was rescued and CDK14 was suppressed. **P < 0.01; ****P < 0.0001