Fig. 2

MKRN1 has a role in CRC cell proliferation, migration, and invasion. A–C WB analysis of MKRN1 transfection rates in HCT116, HT29, and HCT15 cells. D, E CCK-8 assay for CRC cell viability. F, G Colony formation assay to detect CRC cell proliferation. H, I Migration ability of CRC cells with different MKRN1 expression levels detected using a wound healing assay (Scale bar: 100 µm). G-K Transwell assays for migration and invasion of MKRN1 knockdown and overexpressing cells (Scale bar: 50 µm). L, M Microscopic observation of the morphology of the cell lines HCT116 (Control, sh1-MKRN1) and HCT15 (Vector, OE-MKRN1) (Scale bar: 100 µm). N, O Comparison of epithelial and mesenchymal marker expression following knockdown and MKRN1 overexpression. * P < 0.05, ** P < 0.01, *** P < 0.001