Fig. 3

MKRN1 and SNIP1 interaction. A Prediction of protein interaction with MKRN1 using the STRING database. B Prediction of protein interaction with MKRN1 using the IntAct database. C The experimental process of MKRN1 quantitative proteomics and ubiquitination modification omics. D Expression distribution of SNIP1 (upper) and TRA2A (lower) in colorectal tumour and normal tissues. E Univariate and multifactorial Cox analyses of P-values, hazard rate, and confidence intervals for gene expression and clinical characteristics. F, G WB analysis showing that MKRN1 expression level affects SNIP1 protein expression. H Confocal microscopy showing MKRN1 and SNIP1 localisation (Scale bar: 20 µm). I, J Forward and reverse validation of MKRN1 interactions with SNIP1 in HCT116 and HCT15 cells using co-immunoprecipitation (Co-IP) and WB. K Validation of exogenous MKRN1 interaction with SNIP1 in HCT15 cells using Co-IP and WB. *** P < 0.001