Fig. 5

MKRN1 induces EMT in CRC cells by degrading SNIP1 protein. A, B WB was used to determine the transfection rate of SNIP1 in HCT116 and HCT15 cells. C, D WB was used to measure expression levels of epithelial and mesenchymal markers following SNIP1 knockdown and overexpression. E, F Transwell assays for migration and invasion of SNIP1 knockdown and overexpressing cells (Scale bar: 50 µm). G WB was used to determine the level of major EMT proteins in HCT116 cells co-transfected with control, sh1-MKRN1, and sh-SNIP1. H WB analysis of the level of major EMT proteins in HCT15 cells co-transfected with vector, OE-MKRN1, and OE-SNIP1. I Transwell assay of the migration ability of HCT116 cells after co-transfection with control, sh1-MKRN1, and sh-SNIP1 (Scale bar: 50 µm). J Transwell assay of HCT15 cells co-transfected with vector, OE-MKRN1, and OE-SNIP1 for cell migration (scale bar: 50 µm). * P < 0.05, ** P < 0.01, *** P < 0.001