Fig. 7

MKRN1 promotes tumour proliferation and metastasis in vivo. A Comparative graph showing the number of intestinal lesions in the MKRN1 [+ / +] and MKRN1 [f/f] groups. B Haematoxylin–eosin (H&E) staining of the intestine of both groups of mice (scale bar: 100 μm). C H&E staining of the liver in the two groups of mice (scale bar: 100 µm; scale bar: 20 µm). D IHC staining for E-cadherin, MKRN1, SNIP1, and TGF-β1 in the intestinal tissues of the two groups of mice (scale bar: 100 µm). E Western blotting analysis of E-cadherin, MKRN1, SNIP1, and TGF-β1 protein expression in intestinal tissues of the two groups of mice. F MKRN1 facilitates the TGF-β signalling via ubiquitination and degradation of SNIP1, thereby promoting EMT in CRC cells. * P < 0.05, ** P < 0.01, *** P < 0.001