Fig. 2

knockdown of SENP5 increased radiosensitivity in vitro and in vivo. A Western blotting analysis of SENP5 in irradiated HCT116 and HT29 cells at 8 Gy. B-C IF staining of SENP5 at different time after irradiation (8 Gy) in HCT116 and HT29 cells. Scale bar = 10 μm. D Cell viability analysis in irradiated HCT116 cells with NC or SENP5 knockdown transfection. ****P < 0.0001, **P < 0.01 Vs NC transfected cells. E-F Colony formation analysis of radiosensitivity in HCT116 cells with NC and SENP5 knockdown cells after 0, 2, 4 and 6 Gy irradiation. ***P < 0.001 Vs NC transfected cells. G-H Cell apoptosis assay detected with a Annexin V/PI double staining method. **P < 0.01 Vs NC transfected cells. I Representative images of tumors derived from NC and SENP5 knockdown cells after local irradiation. J Growth curve of cell derived xenograft (CDX) in tumors derived from NC and SENP5 KD cells. ***P < 0.0001 Vs relative NC groups. K Immunohistochemistry staining of TUNEL and Ki67 in tumor tissues isolated from NC and SENP5 knockdown tumors after irradiation