Fig. 3

SENP5 is essential for HR-mediated DNA damage repair. A Volcano plot of differentially expressed genes in irradiated NC and SENP5 knockdown HCT116 cells (P < 0.05, |log2FC|≥1). B-C KEGG and GO enrichment of downregulated genes. D-E IF staining and quantitative analysis of γH2AX foci in NC and SENP5 knockdown HCT116 cells after 5 Gy irradiation. Scale bar = 10 μm. ***P < 0.001 Vs relative NC groups. F Representative images of comet assay in irradiated NC and SENP5 knockdown HCT116 cells. scale bar = 50 μm. G Tailed moment analysis of comet assay. ****P < 0.0001 Vs relative NC groups. H-I NHEJ and HR reporter analysis of Isel mediated DNA damage detected by flow cytometry assay. ***P < 0.001 Vs relative NC groups. J-K IF staining and quantitative analysis of Rad51 foci in NC and SENP5 knockdown cells after 5 Gy irradiation. Scale bar = 10 μm. ****P < 0.0001 Vs relative NC groups