Fig. 4

DeSUMOylation function of SENP5 is critical for its role in HR repair and radioresistance. A Schematic structure of SENP5 and the location of C713 site. B Re-expression of SENP5-wt and SENP5 C713L mutant in SENP5 knockdown cells. C-D Cell viability assay and colony formation analysis of with SENP5 knockdown HCT116 cells rescued with wild-type and C713L mutant SENP5. ****P < 0.0001, ***P < 0.001, **P < 0.01, Vs relative SENP5-wt rescued groups. E G2/M cell cycle arrest were detected with flow cytometry in SENP5 knockdown cells rescued with different mutants. Ns, non-significance, ***P < 0.001, Vs relative SENP5-wt rescued groups. F Cell apoptosis was detected in cells rescued with different mutants. Ns, non-significance, ***P < 0.001, Vs relative SENP5-wt rescued groups. G Activation of ATR related HR repair was detected by Western blotting assay in cells rescued with SENP5-wt and C713 mutant. H DNA damage repair efficacy were determined with γH2AX foci in SENP5 cells rescued with SENP5-wt and C713 mutant