Fig. 7

SUMOylation of H2AZ play critical role in SENP5 mediated HR repair. A 3D structure of H2AZ was obtained from the I-TASSER server. And K120, K121 and K126 sites were observed to be responsible for DNA binding. B H2AZ-wt and H2AZ-K3R was constructed and expressed into H2AZ knockdown cells. C Activation of ATR related HR repair was detected by Western blotting assay in cells rescued with H2AZ-wt and K3R mutant. D-E Rad51 foci as key marker of HR repair was detected with IF assay in cells rescued with different mutants. Scale bar = 10 μm. ****P < 0.0001 Vs the H2AZ-wt group. F-G DNA damage were evaluated with comet assay in cells rescued with different mutants. ****P < 0.0001 Vs the H2AZ-wt group