Fig. 1

MYC confers susceptibility of Group 3 MB cells to pharmacological inhibition of CDK12/13. A Representative images of SHH and Group 3 MB cell lines used in this study. Scale bar: 50 μm. B WB analysis of MYC expression level in MB cells shown in A. HSP90 was used as loading control. Blots are representative of 3 independent experiments. C MTS assay of MB cells treated for 72 h with increasing doses of THZ531, as indicated. Data represent mean ± SEM of 3 independent experiments. D Pearson’s correlation between MYC protein expression levels and MB cell viability (%) following treatment with 100 nM THZ531 for 72 h. Pearson’s correlation coefficient (r) and p-value (p) are reported. Data relative to cell viability are reported as mean ± SEM of 3 independent experiments and MYC protein expression is expressed as mean value obtained from densitometric analysis of 3 biological replicates