Fig. 2

Pharmacologic inhibition of CDK12/13 reprograms the transcriptome of MYC-driven Group 3 MB cells. A Heat map of gene expression after treatment with 200 nM THZ531 for 6 h in D341 cell line. FC > 2, p-Adj < 0.05. B Bar graph shows the percentage of regulated and unregulated genes in D341 cells upon treatment with THZ531, as above. C Pie chart of up-regulated and down-regulated genes (%) in D341 cells upon treatment with THZ531, as above. D GO analysis of up-regulated genes performed by using TopGO package in R Studio Software. P-value was calculated with Classic Fisher’s Exact test. E GO analysis of down-regulated genes performed as above. F Bar graphs showing the results of qPCR analyses for the expression of the indicated genes. mRNA expression is normalized on GAPDH expression. Graphs show the mean ± SD of 3 independent experiments. Statistical analysis was performed by unpaired two-tailed Student’s t test (**p < 0.01, ****p < 0.0001)