Fig. 3

CDK12/13 inhibition impairs the DNA damage response in MYC-driven Group 3 MB cells. A Heat map of differentially expressed genes in Group 3 MB patients compared to fetal and adult cerebella. B Venn diagram showing a significant overlap between genes down-regulated in Group 3 MB patients compared to healthy controls and genes up-regulated in THZ531-treated D341 cells, as above. Statistical analysis was performed by hypergeometric test using the phyper function of R Stats Package in R Studio Software. C GO terms enriched for genes of the overlap shown in D. GO analysis was performed as above. D Venn diagram showing a significant overlap between genes up-regulated in Group 3 MB patients compared to healthy controls and genes down-regulated in D341 cells upon treatment with 200 nM THZ531 for 6 h. Statistical analysis was performed by hypergeometric test using the phyper function of R Stats Package in R Studio Software. E GO analysis performed on genes of the overlap shown in B. GO analysis was performed as above. F Boxplot comparing RNA expression of MYC between normal adult cerebellum (n = 5), normal fetal cerebellum (n = 4) and Group 3 MB samples (n = 16) derived from the RNA-seq expression dataset EGAD00001004958. Data were analyzed by the Welch’s t-test (**p < 0.01, ***p < 0.001, ****p < 0.0001). G Venn diagram showing a significant overlap between genes down-regulated in siMYC-depleted D341 cells and upon treatment with THZ531, as above. Statistical analysis was performed by hypergeometric test using the phyper function of R Stats Package in R Studio Software. H GO analysis performed on genes of the overlap shown in G. GO analysis was performed as above