Fig. 6

Chemical inhibition of CDK12/13 induces a massive apoptotic death of MYC-driven Group 3 MB cells. A-F FACS analysis of PI-stained DAOY (A), ONS-76 (B), D283 (C), MED-411 (D), D341 (E) and HD-MBO3 (F) cells following treatment with 100 nM THZ531 for 24 h. Data are represented as mean ± SD of 3 independent experiments. Statistical analysis was performed by One-Way ANOVA test (*p < 0.05, **p < 0.01, ****p < 0.0001). G WB analysis of cleaved PARP and MYC expression in MB cell lines treated for 24 h with THZ531, as indicated. Blots are representative of 3 independent experiments. HSP90 was used as loading control. H Densitometric analysis of the ratio between cleaved and full-length PARP isoforms relative to WB analysis shown in G. Data are represented as mean ± SD of 3 independent experiments. Statistical analysis was performed by One-Way ANOVA test (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001)