Fig. 7

CDK12/13 inhibition reduces the RNAPII processivity within DDR genes in MYC-driven Group 3 MB cells. A Metagene plot showing percentages of read counts per million mapped reads distribution within the gene body from transcription start site (TSS) to transcription end site (TES) of down-regulated (DOWN, green line) and up-regulated (UP, red line) genes in untreated D341 cells. B Metagene plot showing percentages of read counts per million mapped reads distribution of down-regulated genes in DMSO- (light green line) or THZ531-treated (dark green line) D341 cells. C Metagene plot showing percentages of read counts per million mapped reads distribution of up-regulated genes in DMSO- (light red line) and THZ531-treated (dark red line) D341 cells. D Bar graphs showing results of qPCR analyses of the ratio between distal and proximal pre-mRNA region of the indicated DDR genes in DAOY, D283 and D341 cells. Data represent the mean ± SD of 3 independent experiments. Statistical analysis was performed by One-Way ANOVA test (***p < 0.001). E Bar graphs showing results of qPCR analyses of the ratio between distal and proximal pre-mRNA region of DDR genes in DAOY, D283 and D341 cells after treatment with 100 nM THZ531 for 8 h. Data are expressed as fold change over DMSO-treated cell lines set as 1 (red line). Graphs represent the mean ± SD of 3 independent experiments. Statistical analysis was performed by Unpaired t-test (**p < 0.01, ***p < 0.001, ****p < 0.0001)