Fig. 8

Combined treatments with THZ531 and DNA damage-inducing agents show synergistic effect in suppressing cell viability. A-D MTS assay of MB cell lines pre-treated for 8 h with a suboptimal dose of THZ531 (50 nM) or DMSO as control, before administration of DNA damage-inducing drugs, such as Cisplatin (A), Olaparib (B), ATR kinase inhibitor/Ceralasertib (C) and ATM kinase inhibitor (D). Data represent mean ± SD of 3 independent experiments. Statistical analysis was performed by One-Way ANOVA test (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001). The calculated combination index (CI) values for drug combination relative to the individual drugs are presented above the graphs. CI was calculated using CompuSyn software. CI = 1 and < 1 indicates additive effect and synergism, respectively. All results are expressed as the mean ± SD derived from biological triplicates