Fig. 1

circRNA expression profile in NSCLC. A The scatter plot was used for assessing the variation in circRNA expression between tumor and paratumor samples. The values of x and y axes in the scatter plot were the normalized signal values of the samples (log2 scaled). B The volcano plot was constructed using fold-change values and P-values. The red dots represent differentially expressed circRNAs with statistically significant more than 2-fold changes. C The cluster heat map showed the differentially expressed circRNAs over 2-fold change. Red color indicates high expression level, and green color indicates low expression level. D Numbers of identified circRNAs in different chromosomes. E Expression of the top 10 downregulated circRNAs in tissues. F circGUCY1A2 expressions were evaluated using qRT-PCR in 120 pairs of NSCLC tissues and the matched adjacent normal tissues. G Kaplan-Meier survival curve indicated the high circGUCY1A2 expression is correlated with low survival rates. Data were represented as the mean ± SEM of three independent experiments. H The location of circRNA in chromatin, and its loop-forming site status I Total RNA extracted from the negative control or circGUCY1A2- transfected A549 and H1703 cells was incubated with or without RNAse R followed by real-time PCR. RNAse R treatment did not affect circGUCY1A2 levels but decreased linear GUCY1A2 mRNA levels. Ns: no significance, *P < 0.05, **P < 0.01, ***P < 0.001. Error bars indicate the S.E.M