Fig. 3

circGUCY1A2 affects NSCLC apoptosis by binding miR-200c-3p A Relative expression of the five indicated miRNAs from 20 NSCLC tumor tissues and the matched adjacent normal tissues. T: tumor; N: nontumor. B The expressions of miR-200c-3p were analyzed using qRT-PCR in NSCLC cells transfected with negative control or pLCDH-circGUCY1A2. C Schematic of circGUCY1A2 wild-type (WT) and mutant (Mut) luciferase reporter vectors. The relative luciferase activities were analyzed in A549 cells co-transfected with miR-200c-3p mimics or miR-NC and luciferase reporter vectors pLCDH-circGUCY1A2- WT or pLCDH-circGUCY1A2- Mut. D AGO2 RNA immunoprecipitation (RIP) assay for circGUCY1A2 and miR-200c-3p levels in NSCLC cells. E miR-200c-3p co-localized with cirR-GUCY1A2 in H1299 was detected by FISH, Magnification, 400×. F After overexpression of circGUCY1A2, miR-200c-3p mimic was added to A549 and H1703 cell culture. Apoptosis ratio of NSCLC cells was measured by fluorescent-activated cell sorting. Data were represented as the mean ± SEM of three independent experiments. Ns: no significance, *P < 0.05, **P < 0.01, ***P < 0.001. Error bars indicate the S.E.M