Fig. 4

miR-200c-3p affects lung adenocarcinoma through regulation of PTEN. A-B After overexpression of circGUCY1A2, miR-200c-3p mimic was added to A549 and H1703 cell culture. Proliferation ratio of NSCLC cells was measured by EdU incorporation. C Location and structural information of miR-200c-3p. D Correlation analysis between miR-200c-3p and PTEN expression in patients with lung adenocarcinoma. E Schematic of the construction of wild-type or mutant pGL3-PTEN 3’-UTR vectors is illustrated. Relative luciferase activity was analyzed in A549 cells. Firefly luciferase vector was used as an internal control. F Inhibiting miR-200c-3p and knocking out PTEN to observe the protein level of PTEN/PI3K/AKT. G After overexpression of circGUCY1A2, miR-200c-3p mimic was added to A549 and H1703 cell culture. The PTEN protein expression level was analyzed by Western blot. Data were represented as the mean ± SEM of three independent experiments. Ns: no significance, *P < 0.05, **P < 0.01, ***P < 0.001. Error bars indicate the S.E.M