Fig. 6

ARNTL can initiate transcription of circGUCY1A2. A Identification of tumor-specific and prognostically relevant transcription factors through GTRD and TCGA databases. B Forest plots of core tumor-specific and prognostically relevant transcription factors and clinical indicators. C ARNTL overexpression in A549 and PC-9 can significantly increase circGUCY1A2 expression. D ARNTL expressions were evaluated using qRT-PCR in NSCLC tissues and the matched adjacent normal tissues. E Kaplan-Meier survival curve indicated the low ARNTL expression is correlated with low survival rates. Data were represented as the mean ± SEM of three independent experiments. F Prediction of ARNTL three binding sites and motif to GUCY1A2. G CHIP-qPCR results suggest that ARNTL may bind to promoter and promoter 2. H Gel electrophoresis was performed to demonstrate information of DNA sonication interruption and qRT-PCR products. Ns: no significance, *P < 0.05, **P < 0.01, ***P < 0.001. Error bars indicate the S.E.M