Fig. 1

FTO is associated with gemcitabine resistance. A FTO and ALKBH5 expression in gemcitabine-resistant and -sensitive cells was evaluated by western blot. B Expression level of FTO mRNA in gemcitabine-resistant and -sensitive cells. C The findings of the CCK-8 proliferation experiment showed that inhibiting FTO expression reduced the growth of gemcitabine-resistant cells. D The ability of CFPAC-GM and Colo357-GM cells to form colonies was inhibited by decreased FTO expression. E Cell-cycle profiles of CFPAC-GM and Colo357-GM cells after FTO knockdown. F The chemosensitivity of CFPAC-GM and Colo357-GM cells to gemcitabine was assessed using Cell Counting Kit-8 (CCK-8) toxicity tests (GEM). The IC50 values were calculated. G, H Silencing FTO in CFPAC-GM cells inhibited pancreatic tumor growth in nude mouse models