Fig. 5

NEDD4 promotes the proliferation of gemcitabine-resistant cells A NEDD4 expression in gemcitabine-resistant and -sensitive cells was evaluated by Western blot. B CCK8 proliferation assay in cells with shNEDD4 and shFTO with NEDD4 vector. C Colony formation assay of NEDD4. D Cell-cycle profiles of CFPAC-GM and Colo357-GM cells after the NEDD4 knockdown and rescue experiment. E The chemosensitivity of CFPAC-GM and Colo357-GM cells to gemcitabine was assessed using CCK-8 toxicity tests. The IC50 values were calculated. F, G Silencing NEDD4 in CFPAC-GM cells inhibited pancreatic tumor growth in nude mouse models. The overexpression of NEDD4 rescued the tumor growth rate caused by shFTO. H, I Silencing FTO or overexpressing NEDD4 in CFPAC-GM cells influenced pancreatic tumor growth in nude mouse models treated with gemcitabine. Group A: cells transfected with shFTO-NC. Group B: cells transfected with shFTO-1. Group C: cells transfected with both shFTO-1 and NEDD4 overexpression plasmid. Group D: cells transfected with shFTO-1 and NEDD4 control vector