Fig. 6

FTO promotes the gemcitabine sensitivity of PDAC cells by regulating the PTEN/AKT/PI3K pathway. A Endogenous protein interactions in HEK 293 T lysates were verified by immunoprecipitation with anti-PTEN or anti-NEDD4 followed by immunoblotting with anti-NEDD4 or anti-PTEN, respectively. B The levels of the NEDD4 and PTEN proteins in HEK 293 T cells transfected with shNEDD4 or HA-NEDD4 plasmid were analyzed using western blot. C After MG132 treatment and shCtrl or shNEDD4 transfection, lysates from HEK 293 T cells were harvested. The cells were then immunoprecipitated and the results were analyzed using the appropriate antibodies. D, E The protein levels of PTEN, p-PI3K, p-AKT were detected by western blot in CFPAC-GM and Colo357-GM cells with the indicated treatments