Fig. 6

The combination of RBA plus ASC acid significantly prolongs the life span of NSG mice engrafted with human FLT3-ITD⁺ MOLM-13 cells. A Kaplan-Meyer survival plot showing OS of NSG mice engrafted with MOLM-13 leukemic cell line and treated with vehicle, RBA, or RBA + ASC, starting at day 2 after cell injection. (control = 12, RBA = 5 and RBA + ASC = 17 from three independent experiments). B Histological analysis of the spleen, liver, and kidney of NSG engrafted mice, treated or not with the combination RBA plus ASC, show no signs of toxicity due to the treatment. C The treatment with RBA plus ASC reduces the percentage of leukemic cells in the BM of engrafted mice. Both control and treated mice were sacrificed when showing posterior limb paralysis. Bone marrow was flushed by femurs and purified mononucleated cells were analyzed by flow cytometry after staining with an anti-human CD45 antibody (unpaired Student’s T test). D Wright-Giemsa staining of the cells collected from mice bone marrow shows extensive vacuolation of MOLM-13 cells isolated from the BM of treated mice present to a much lesser extent in those obtained from control ones. G indicates murine granulocytes that show no vacuolation