Fig. 5

Evaluation of macrophage changes in vivo and exploration of the mechanism. A Experimental layout. BALB/c mice were subcutaneously (s.c.) inoculated with 2 × 10⁵ CT26 cells in the right flanks, smTRAIL was injected intraperitoneally (i.p.) for eight consecutive days, and each mouse was intravenously (i.v.) treated with 50 μg/kg clodronate liposomes every four days (n = 6). B Tumor growth curves. C UMAP plot of reclustered macrophage cells from Fig. S4A. D Expression of key genes used for identification of cell clusters from (C) by violin plot. E Stacked histograms of the frequencies of macrophage cell clusters in the groups. F Trajectory plot of macrophage clusters. G GOBP analysis performed in all macrophage cells comparing 2 mg/kg versus 8 mg/kg. H Violin plot showing the expression of Ccr2 in macrophage cell clusters. A two-way ANOVA test was used to compare the tumor growth responses. **P < 0.01; and ***P < 0.001