Fig. 3

Association of signaling responsiveness in regulatory T cell (Treg) II cells with intratumoral Tregs and suppressive function. Peripheral Treg populations were defined based on differential expression of CD45RA and FoxP3 and compared to intratumoral Tregs A. Peripheral Treg populations were compared to intratumoral Tregs for expression of CD25 and other markers (not shown) B. T cell receptor sequencing was performed and the proportion of overlapping clones among the top 50 clones was compared between peripheral Treg populations and matched intratumoral Tregs C. A cytokine signaling index (CSI) was calculated from the z-score of the difference in median fluorescent intensities of cytokine-stimulated minus unstimulated groups D. Suppression of responder T cells (CD4⁺CD45RA⁺CD25⁻) by Treg I, II, and III cells was compared, and Treg II cell suppression was correlated with the Treg II CSI E. Modified from Wang et al., Nat Immunol. 2019 [47]. Copyright© 2019, Springer Nature