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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: CHSY1 promotes CD8+ T cell exhaustion through activation of succinate metabolism pathway leading to colorectal cancer liver metastasis based on CRISPR/Cas9 screening

Fig. 2

CHSY1 facilitates the proliferation, invasion and migration of CRC cells. A, B Three shRNAs (sh1, sh2, and sh3) were designed to silence CHSY1 in CRC cells (HCT116 and LOVO), and validated by qRT-PCR and western blotting. C EdU assay was performed to assess cell proliferation of CRC cells transfected with sh3-CHSY1/sh-NC. D The growth curves of cells were plotted after transfection with sh3-CHSY1/sh-NC based on CCK-8 assay. E Transwell invasion and migration assay were performed to assess invasion and migration of CRC cells transfected with sh3-CHSY1/sh-NC.Scale bar, 100 μm. F Wound Healing assay was performed to assess migration of CRC cells transfected with sh3-CHSY1/sh-NC.Scale bar, 100 μm. G-HThree shRNAs (sh1, sh2, and sh3) were designed to silence CHSY1 in MC38 cells, and validated by qRT-PCR and western blotting. *, P < 0.05; **, P < 0.01; ***, P < 0.001;****, P < 0.0001

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Journal of Experimental & Clinical Cancer Research

ISSN: 1756-9966

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