Fig. 3

Expression profiles of CHSY1 and PD-L1 in human tissue samples and their interactions in human CRC cell lines. A, B Immunofluorescence detection of CHSY1 and PD-L1 expression in primary and secondary lesions and corresponding paracancerous tissues of 4 patients with CRC liver metastases. C, D qRT-PCR and western blotting were performed to detect PD-L1 expression in human CRC cells after CHSY1 knockdown.**, P < 0.01; ***, P < 0.001;****, P < 0.0001