Fig. 3

Role of circulating EVs in tumor cell invasion and migration ability and in neoangiogenesis. A Scatter plots with median reporting the number of invasive MM cells (BRAF wt or mutated) in presence or absence of circulating EVs from NRES, long RES and RES > PRO (before and after ICI) (**p < 0.01). B Bar plot showing the % (mean ± SD) of migrated LND-1 cells, as respect to control without EVs, towards M0 in presence of circulating EVs from NRES, long RES and RES > PRO (before and after ICI). C Representative images of H-MVECs in vitro capillary morphogenesis in presence or absence of circulating EVs from long RES (before and after ICI) (scale bar = 200 µm). D Bar plots reporting the amount of VEGF and IL-1β release (pg/mL) from M0 in presence or absence of circulating EVs from NRES, long RES and RES > PRO (before and after ICI), quantified by Bioplex assay. (*p < 0.05, **p < 0.01, ***p < 0.001)