Fig. 3

Identification of downstream pathways of IGF2BP1 in B-ALL. A) Western Blotting after RNA Immunoprecipitation of IGF2BP1 in Reh cell line; Specificity of the immunoprecipitation established by Western blotting analysis for another family member, IGF2BP3. Input is the precleared Reh cell lysate; L: Ladder; IgG: Mouse IgG; IP: IGF2BP1 Pulldown B) Output of EnrichR package used to categorize the IGF2BP1 RIP targets using GWAS catalogue C) Volcano plot of IGF2BP1 RIP-Seq data in Reh cell line. Genes up and down-regulated (RNA-Seq) after IGF2BP1 KO are highlighted as black and red dots respectively D) GSEA analyzing the association between enrichment in IGF2BP1 RIP-Seq samples and genes regulated in IGF2BP1 KO cells. X axis represents the pre-ranked list of genes based on RIP-Seq PC1 loadings, which segregates between genes enriched (right) and depleted (left) in IP samples. Segment plots (bottom) highlight the position of genes strongly up/down regulated in IGF2BP1 KO cells. The vertical axis in line plots (top) represents the cumulative Enrichment Score (ES) from GSEA, and NES is the overall normalized enrichment score (with FWER=familywise error rate) for each gene set E) Pathway enrichment results for several gene classes: genes strongly up/down regulated in IGF2BP1 KO cells and genes strongly enriched or depleted in the IGF2BP1 RIP-Seq dataset. For each pathway, shown are the hypergeometric adjusted p-values for each gene class