Fig. 4

Functional enrichment analysis of IGF2BP1 regulated pathways in B-ALL. A) Rank distribution plots of genes in selected functional categories. For each pathway, the x-axis represents the gene’s rank based on RNA-Seq PC1 loadings, which segregates between genes more expressed in IGF2BP1 KO cells (right) and wild type cells (left). The y axis represents the gene’s rank based on RIP-Seq PC1 loadings, which positions RIP-enriched genes on top, and depleted genes at the bottom. Shown are selected gene names of top-ranked genes in each pathway B) GSEA results of leukemia-associated genes in IGF2BP1 KO Reh cells. X axis represents the pre-ranked list of genes based on PC1 loadings, which segregates between genes more expressed in IGF2BP1 KO cells (left) and wild type cells (right). Segment plots (bottom) highlight the position of genes from several genesets identified as up/down regulated in two independent studies comparing ETV6::RUNX1 with KMT2A and TCF3::PBX1 rearranged leukemias. The vertical axis in line plots (top) represents the cumulative Enrichment Score (ES) from GSEA, and NES is the overall normalized enrichment score (with FWER=familywise error rate) for each gene set. Color-coded names for some genes in selected gene sets are shown