Fig. 3

Distinct functional GSCs subsets might be discriminated within GBM subtypes. A Phase-bright microphotographs of neurospheres from classical, mesenchymal and proneural GSCs cultures and human neural stem cells (NSCs). Bar: 100 µm. B In vitro migration assays reporting the higher degree of invasiveness in TCGA-MS GSCs versus their TCGA-CL and PN counterpart and normal neural stem cells. Bar: 50 µm (Top). Quantification is shown as mean ± SEM (bottom). P-values from ANOVA multiple comparison test are reported. C-D Analysis of long-term (C) and short-term (D) proliferation showing that mesenchymal and proneural GSCs (blue and green lines, respectively) are identified by an inherent expansion rate and a self-renewal capacity significantly much lower than that one of classical GSCs (red lines) (hierarchical linear model for repeated measurements). Slope’s value and confidence interval (CI) are shown