Fig. 4

GSCs populations are inherently endowed with different tumorigenic and lethal potentials. A Quantitative time-course imaging analysis demonstrating that GBM xenografts from classical (shades of red lines; left), mesenchymal (shades of blue lines; middle) and proneural (shades of green lines; right) GSCs display heterogeneous tumor growth with the former being faster than the others. Error bars indicate mean ± SEM. Slope’s value and CI are shown. B Imaging of luciferase-tagged TCGA-CL (left), MS (middle) and TCGA-PN (right) GSCs injected into the brain of SCID mice from 7–10 DPT next to the end-stage disease typical of each subtype injected. C Immunohistochemical reconstruction analysis of brain samples from the experiment in A demonstrating that spreading of those tumors established by mesenchymal and proneural GSCs, with cells that infiltrated extensively from the transplantation site within the contralateral hemisphere is significantly enhanced as compared to classical GSCs-tumors, which display more compact masses. (ST: striatum; CC: corpus callosum; LV: lateral ventricle). Bars, 100 um and 1 mm. D Kaplan–Meier plots of overall survival showing that mice transplanted with mesenchymal GSCs (n = 20; blue lines; median = 132 days) survived longer than those implanted with classical (n = 20; red lines; median = 64 days) and proneural (n = 17; green lines; median = 101 days) lines. P-values from Log-rank and Gehan Breslow-Wilcoxon tests are shown