Fig. 1

Oxamate enhanced the function of TILs in both control T and CAR-T cells inoculation. 51 A GSCs were orthotopically inoculated into nude mice to establish glioma model. The tumor bearing mice were injected with sodium oxamate with or without control T or CAR-T cells. Tumors were removed from the mice at day 28, then prepared into sections for IHC staining or single cell suspensions for flow cytometry assay. (A) Timeline of tumor-bearing mouse models with treatment schedule. (B) Representative images of formalin-fixed paraffin embedded sections from xenografts stained using anti-CD4 and CD8 antibodies (×200). Scale bar represents 50 μm. (C) All lymphocytes were gated as CD45 + CD3 + cells. The percentages of CD4 + and CD8 + T cells within the CD45 + cells and CD4 + CD25 + T cells within the CD4 + cells. (D) The median fluorescence intensity (MFI) of perforin, granzyme B, and IFN-γ in CD8 + T cells. N = 5, *P < 0.05, **P < 0.01