Fig. 2

Oxamate unchanged myeloid subpopulation in tumor tissue while reduced tumor growth. (A-C) 51 A GSCs were orthotopically inoculated into nude mice and tumors were removed from the mice at day 28. (A) Tumors were dissociated into single cells for flow cytometry assay. The classification of myeloid subpopulations was gated in CD45 + CD11b + cells. The percentages of TAMs (CD11b+), macrophages (CD11b + F4/80+), and M-MDSCs (CD11b + Ly6C+) within CD45 + cells in tumor tissues were analyzed. (B) Paraffin sections from the whole brain were stained with H&E and representative images showed tumor location and size. Scale bar represents 100 μm. (C) Survival of tumor-bearing nude mice. (D-F) Nude mice with subcutaneous tumor were injected with saline, sodium oxamate, CAR-T or sodium oxamate plus CAR-T combination. (D) Timeline of tumor-bearing mice with treatment schedules. (E) Subcutaneous tumor size in different time points after GSCs implantation. (F) Kaplan–Meier curves showing mice survival. N = 5, *P < 0.05, **P < 0.01