Fig. 4

Lactate increased CD73 expression by H3K18la in GSCs. (A) CD73 levels in tumor and T cells within the xenografts of nude mice. N = 5. GSCs were treated with PBS, 10 mM lactic acid, 10 mM sodium lactate, 2 mM 2-DG or 20 mM sodium oxamate for 24 h, H3K18 lactylation using western blot (B), H3K18la enrichment on CD73 promotor using ChIP (C), CD73 protein level using flow cytometry (D), CD73 mRNA expression using RT-qPCR (E), and intracellular lactate production using colorimetric assay (F) were measured. (G) GSCs were transfected with pGL3-CD73 promotor plasmid for 24 h, then treated with PBS, sodium lactate or sodium oxamate, the luciferase activities were measured by dual-luciferase reporter system. T cells were mono-cultured or co-cultured with GSCs for 12 h, H3K18la levels (H), CD73 mRNA expression (I) and H3K18la enrichment on CD73 promoter region (J) in T cells were detected. (K) The correlation of LDHA and NT5E, editing CD73 protein, expressions in TCGA database. *P < 0.05, **P < 0.01