Fig. 2

CircRNF10 promotes GSCs proliferation and stemness maintenance in a ferroptosis-defending manner in vitro. a Relative cell viability of SYNS03 treated with DMSO, Nec (20 μM), Z-VAD (20 μM), 3-MA (60 μM), and Fer-1(10 μM) for 24 h. b, c GSH(b) and MDA(c) contents measured in SYNS03 and SYNS08 with circRNF10 knockdown followed by Fer-1 treatment. d, e Lipid peroxidation levels (d) detected by BODIPY (581/591) C11 probe in SYNS03 and SYNS08 and the relative fluorescence intensity of O-BODIPY (e) quantified by Image J. Scale bar = 100 μm. f Morphology of mitochondria under transmission electron microscopy after circRNF10 knockdown in SYNS03 and SYNS08. Scale bar = 5 μm. g Representative images of NSFA with circRNF10 knockdown in SYNS03 and SYNS08 followed by Fer-1 treatment. Scale bar = 200 μm. h, i ELDA of neurospheres formation ability in SYNS03 (h) and SYNS08 (i) after circRNF10 silencing followed by Fer-1 treatment. j Relative sizes of neurospheres of circRNF10-silenced SYNS03 and SYNS08 followed by Fer-1 treatment. k Western blotting of the stemness markers expression with circRNF10 knockdown in SYNS03 and SYNS08 followed by Fer-1 treatment. l Representative images of cell death in SYNS03 and SYNS08 after 48 h of indicated treatments. Scale bar = 100 μm. m, n Real-time analysis of cell death in SYNS03 (m) and SYNS08 (n) with circRNF10 knockdown followed by Fer-1 treatment. Data are shown as the mean ± SD (three independent experiments). *p < 0.05; **p < 0.01; ***p < 0.001; ns, no significance