Fig. 2

Fn facilitates NETs generation in vitro. A Representative IF images for DNA/NE/MPO in neutrophils treated with Fn or PMA for 4 h. B Western blot analysis of CitH3 expression in neutrophils treated with Fn or PMA for 4 h. C CitH3 protein levels were quantified by using densitometry and normalized to β-actin and are shown as fold changes compared to the control. Each bar displays the means ± SD of three independent experiments. D ELISA analysis of MPO-DNA levels in the supernatant of neutrophils treated with Fn or PMA for 4 h. None, no treatment. White scale bars: 50 μm. ***p < 0.001