Fig. 1

Identification of targets to inhibit the growth and survival of ovarian cancer TICs. A The top 20 genes identified from an siRNA functional screen that were critical spheroid viability compared to adherent cells using the Z score to compare viability. B RNA-seq data of OV90 cells cultured as spheroids or cultured adherently from GEO accession number GSE158949. Candidate genes were graphed for gene expression on the x axis and p-value on the y axis. C mRNA expression of five candidate genes in Ovarian Serous Cystadenocarcinoma from the Cancer Genome Atlas (TCGA). D Quantification of protein expression of 3 candidate genes in normal ovarian tissue and ovarian carcinomas from the Human Protein Atlas (THPA). ** p <0.01.  E Normalized Enrichment Scores (NES) for hallmarks UGDH and NAMPT were included in from GEO accession number GSE158949, comparing ovarian cancer spheroids and adherent cells by gene set enrichment analysis (GSEA). F Venn diagram to summarize identified target, UGDH