Fig. 5

Effects of UGDH knockdown in C1/MES cell lines, and over-expression in C4/DIF cell lines on adherent and spheroid growth in vitro. Western blot and densitometry analysis of UGDH expression in C1/MES cell lines (A) OV90 cells, and (B) HEYA8 cells, with DOX-inducible negative control shRNA (shneg) or DOX-inducible shRNA targeting UGDH (sh459, sh939) after 3 days of DOX induction in adherent (Adh.) or spheroid (Sph.) culture conditions. Western blot and densitometry analysis of UGDH expression in C4/DIF cell lines: C ACI23 cells, and (D) SKOV3 cells with stably expressed vector control (VC) or UGDH (Ov, OverX) grown for 3 days in, and adherent (Adh.) or spheroid (Sph.) culture conditions. Viability of C1/MES cells grown in adherent or spheroid conditions with control (shneg) and UGDH-knockdown (sh459,sh939): E OV90, F HEYA8. Viability of C4/DIF cells grown in adherent or spheroid conditions with control (VC) and UGDH overexpression (OverX): G ACI23, H SKOV3. Sphere forming capacity of C1/MES cell lines with control (shneg) or UGDH knockdown (sh459, sh939) in (I) OV90 and (J) HEYA8. Sphere forming capacity of C4/DIF cell lines with control (VC) or UGDH overexpression (OverX): K ACI23, L SKOV3. n.s non-significant, *p < 0.05, **p < 0.01, ***p < 0.001