Fig. 6
Effects of UGDH knockdown in C1/MES cell lines, and over-expression in C4/DIF cell lines on TIC populations and functions in vitro. Colony forming capacity of C1/MES cell lines with UGDH knockdown (sh459, sh939) compared to control (shneg) in: A OV90, B HEYA8. Colony-forming capacity in of C4/DIF cell lines with control (VC) and UGDH overexpression (OverX): C ACI23, D SKOV3. Quantification of the proportion of CD133 + ALDH + cells in C1/MES cell lines with UGDH knockdown (sh459, sh939) compared to control (shneg) in: E OV90 and (F) HEYA8. Quantification of the proportion of CD133 + ALDH + population in C4/DIF cell lines with control (VC) and UGDH overexpression (OverX) in (G) ACI23, H SKOV3. Analysis of cell death by AnnexinV and PI double positive cell population from in vitro relapse model in spheroids generated from viable cells collected after 48 h of carboplatin treatment followed by induction of UGDH silencing (sh459, sh939) compared to control (shneg) in C1/MES cells (I) OV90, J HEYA8 or with UGDH overexpression (OverX) or control (VC) in C4/DIF cell lines in (K) ACI23, L SKOV3. n.s non-significant, *p < 0.05, **p < 0.01, ***p < 0.001