Fig. 8

Overall survival, tumor size and histomorphology of xenografts of OV90 with UGDH knockdown, and ACI23 with UGDH overexpression. A Survival analysis of OV90 control (shneg) or UGDH knockdown (shUGDH) intrabursal xenografts. B Cumulative totals of mice with visible tumors at necropsy, at indicated timepoints. Intrabursal xenografts of OV90 control (shneg) or UGDH knockdown (shUGDH) cells were injected and allowed to establish for 7 days prior to DOX induction of shRNAs. C Survival analysis of ACI23 control (VC) or UGDH overexpression (OverX) intrabursal xenografts. D H&E images of OV90 xenografts (left) and quantification of tumor size (right). Tumor is marked by dashed lines; ovary is marked Ov. Scale bar is 3 mm. E H&E images of ACI23 xenografts (left) and quantification of tumor size (right). Scale bar is 3 mm. F Massons trichrome staining images of OV90 xenografts (left) and quantification of collagen in the tissue (right), scale bar is 3 mm for lower power image, 300 µm for inset higher power image. G Massons trichrome staining images of ACI23 xenografts (left) and quantification of collagen in the tissue (right), scale bar is 3 mm for lower power image, 300 µm for inset higher power image. H Expression of VCAN, I LAMA3 (J) IL6 and (K) Nos2 mRNA in ACI23 control (VC) and UGDH overexpressing (OverX) xenograft tumors. n.s non-significant,*p < 0.05, **p < 0.01