Fig. 2

CCAT1 promotes the proliferation, migration and invasion of GC cells in vitro. A GC cells with CCAT1 knockdown or overexpression. B The proliferation, C migration and D invasion were decreased in GC cells with CCAT1 knockdown and those properties were increased in GC cells with CCAT1 overexpression. E The stable CCAT1 knockdown GC cells were established using lentiviral shRNA. F The cell cycle was arrested in G0/G1 phase, and the number of cells in S phase was significantly decreased in CCAT1 knockdown group. G The apoptosis rate was increased in GC cells with CCAT1 knockdown. All cells were imaged at 10 \(\times\) magnification using a Nikon Ti2 Eclipse inverted microscope. All data are presented as the mean values ± SD from three independent experiments. Data were statistically evaluated using the Multiple unpaired t-tests (A, C, D and G), 2-way ANOVA with Tukey's multiple comparisons test (B) and 2-way ANOVA with Sídák's multiple comparisons test (F). ^*p < 0.05, ^**p < 0.01